Fig. 3

Mini-myotube template derivation. A Schematic of the mini-myotube template derivation workflow. Cellulose scaffolds were pre-adsorbed with thrombin and seeded with human pMBs mixed in an ECM mixture containing DMEM, fibrinogen and Geltrex™. B Representative confocal images of SAA (magenta) immunostaining for mini-myotube templates generated with the 18M and 19F cell lines and analyzed on days 7, 10, and 14 of differentiation. Scale bar, 500 \(\upmu\)m. C, D Quantification of SAA coverage in mini-myotube templates generated with the 18M (C) and 19F (D) cell lines on days 7, 10, and 14 of differentiation. Graphs display mean ± s.e.m. one-way ANOVA with Tukey post-test. * p < 0.05, ** p < 0.01 ns. = no significance. n = 6–10 tissues from N = 3–4 independent experiments per cell line