Fig. 4

Myotoxin-induced mini-myotube templates injury. A Schematic of the template injury workflow. Mini-myotube templates are incubated in MEndR differentiation media containing 0.5 \(\upmu\)m cardiotoxin (CTX) for 4 h at 37 °C on an orbital shaker. B Representative confocal images of uninjured (left; (-)) and injured (right; ( +)) mini-myotube templates generated with the 18M (top) or 19F (bottom) cell lines (SAA; magenta) at 1 DPI. Scale bar, 500 \(\upmu\)m. C, D Quantification of SAA tissue coverage in uninjured and injured mini-myotube templates generated with 18M (C) or 19F (D) cell lines at 1 DPI.Graphs display mean ± s.e.m. Unpaired student’s t-test, **** p < 0.0001. n = 11–20 tissues from N = 5–6 independent experiments per cell line