Fig. 5
From: Mini-MEndR: a miniaturized 96-well predictive assay to evaluate muscle stem cell-mediated repair
p38α/β MAPKi induces mini-myotube template self-repair. A Experimental timeline to study mini-myotube template self-repair capacity. B Representative confocal images of uninjured (CTX(-)) and injured (CTX( +)) myotube templates generated with 18M (top) or 19F (bottom) cell lines in response to treatment with p38α/β MAPK inhibitor (p38i) or DMSO carrier control at 10 DPI. Scale bar, 500 \(\upmu\)m. C, D Quantification of SAA coverage in uninjured and injured myotube templates generated with 18M (C) or 19F (D). Graphs display mean ± s.e.m. one-way ANOVA with Tukey post-test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 10–15 tissues from N = 4–5 independent experiments per cell line