Fig. 1

Schematic summary of EV separation and downstream analyses. Following EV separation using five different methods, a comprehensive characterisation was performed by nanoparticle tracking analysis, Western blot, transmission electron microscopy, single particle interferometric reflectance imaging sensing (Leprechaun® analysis) and proteomics (mass spectrometry). Leprechaun® image reproduced with the permission of Unchained Labs (Pleasanton, Ca, USA)