Fig. 2

Comparison of the yield from the five different plasma EV separation methods from a starting sample of 1mL plasma. A BCA™ assay identified the total protein level of the samples from the five different separation methods. b Nanoparticle tracking analysis identified the number of particles separated from the samples. c Sample purity as assessed by the particle to protein ratio. Data presented as mean ± SEM; n = 9 (3 per individual, individuals mean highlighted by symbol), p-values ns not significant, * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001