Table 1 Comparing the common EV associated proteins identified from Karimi et al. [50] with the different separation methods used in this study

Rabs

Rab-1A, -1B, -2A, -2B, -4A, -4B, -5A, -5B, -5C, -6A, -6B, -7a, -8A, -8B, -10, -11B, -14, -18, -21, -27B, -30, -32, -33A, -35, -37, -38

Rab-10, -27B

Rab-10, -27B

Rab-10

Rab-11B

Annexins

Annexin A2, Annexin A4, Annexin A7, Annexin A11

Annexin A11

Annexin 2, Annexin A7, Annexin A11

Annexin A4, Annexin 7, Annexin A11

Tetraspanins

CD9, CD63, CD81, CD82, CD151, TSPAN2, TSPAN14, TSPAN32

CD9

CD9

CD9

Common EV markers

MHC class I, Ezrin, Flotillin-1, Flotillin-2, Cofilin-1, Profilin-1, CD59, 14–3-3 protein (beta/alpha, epsilon, eta, gamma, sigma, theta, zeta/delta)

MHC class 1, Flotillin-1, Cofilin-1, Profilin-1, 14–3-3 protein (beta/alpha, epsilon, eta, gamma, zeta/delta)

MHC class 1, Cofilin-1, Profilin-1, 14–3-3 protein (zeta/delta)

14–3-3 protein (zeta/delta)

MHC class 1, Cofilin-1, Profilin-1, 14–3-3 protein (beta/alpha, epsilon, eta, zeta/delta)

Flotillin-1, Flotillin-2, Cofilin-1, Profilin-1, 14–3-3 protein (zeta/delta)

Heat shock proteins

Heat shock 70 kDa protein 1A/1B, Heat shock cognate 71 kDa, Heat shock protein 75 kDa (mitochondrial), Heat shock protein beta-1, Heat shock protein HSP 90-alpha and beta

Heat shock cognate 71 kDa, Heat shock protein HSP 90-alpha and beta

Heat shock cognate 71 kDa,

Heat shock cognate 71 kDa, Heat shock protein HSP 90-alpha and beta

Heat shock cognate 71 kDa, Heat shock protein HSP 90-alpha and beta

ESCRT

VPS-28, VPS-37B, CHMP4B, CHMP6, Clathrin, Alix

Clathrin