Fig. 2

SLAM-seq detects global transcriptional changes associated with rapid depletion of Bdf1/2. A Scatterplot comparing log2 fold change in transcription and log10 baseline transcription following Bdf1/2 depletion (strain RDY73) across the indicated number of transcripts (n = 5311). Total read counts were normalized using size factors calculated on total read counts from exogenous whole cell spike-in. Mean values for three biological replicates are plotted. B Scatterplot comparing log2 fold change in transcription and log10 baseline transcription following Bdf1/2 depletion (strain RDY73) across the indicated number of transcripts (n = 5311). T > C read counts were normalized using size factors calculated on total read counts from exogenous whole cell spike-in. Mean values for three biological replicates are plotted. C Boxplots comparing log2 fold changes in transcription following Bdf1/2 depletion (strain RDY73) normalized using exogenous whole cell spike-in. Mean changes in transcription for three biological replicates across 5311 transcripts are presented as Tukey boxplots. D Scatterplot comparing log2 fold change in transcription and log10 baseline transcription following Bdf1/2 depletion in a strain ectopically expressing BDF1 (RDY234) across the indicated number of transcripts (n = 5311). Total read counts were normalized using size factors calculated on total read counts from exogenous whole cell spike-in. Mean values for three biological replicates are plotted. E Scatterplot comparing log2 fold change in transcription and log10 baseline transcription following Bdf1/2 depletion in a strain ectopically expressing BDF1 (RDY234) across the indicated number of transcripts (n = 5311). T > C read counts were normalized using size factors calculated on total read counts from exogenous whole cell spike-in. Mean values for three biological replicates are plotted. F Boxplots comparing log2 fold changes in transcription following Bdf1/2 depletion in a strain ectopically expressing BDF1 (RDY234) normalized using exogenous whole cell spike-in. Mean changes in transcription for three biological replicates across 5311 transcripts are presented as Tukey boxplots