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Fig. 2 | BMC Methods

Fig. 2

From: Scalable isolation of surface-engineered extracellular vesicles and separation of free proteins via tangential flow filtration and size exclusion chromatography (TFF-SEC)

Fig. 2

Characterization of eEVs isolated by TFF and centrifugation. A Particle concentrations per 1 mL of media isolated by each MWCO TFF or centrifugation EVs from TFF filtrate were collected by ultracentrifugation. B The DNA copy number per µL of EVs quantified by qPCR before and after DNase I treatment. C The DNA copy number per particle following DNase I treatment for each fraction. D Western blot analysis of entire particles of eEVs isolated from 8mL of conditioned media. EV marker – Alix and CD63, and engineered protein marker – HA. A-C were performed n = 3. Error bars represent standard deviation

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BMC Methods

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