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Fig. 3 | BMC Methods

Fig. 3

From: Scalable isolation of surface-engineered extracellular vesicles and separation of free proteins via tangential flow filtration and size exclusion chromatography (TFF-SEC)

Fig. 3

Analysis of eEVs purified further by Size Exclusion Chromatography (SEC) following TFF concentration. 1 mL of TFF (between 50 and 650 nm) isolated EVs were loaded into the SEC column. Each 1 mL fraction was collected and analyzed. A Particle concentration and Protein concentration of Naïve EVs (B) Particle concentration and protein concentration of engineered EVs (RDG-EVs). C Particle concentration and protected DNA amount of engineered EVs (RDG-EVs). This experiment was performed n = 3, and one of the data was presented. D Particle concentration and protected DNA amount of engineered EVs (RDG-EVs) that were isolated by Ultracentrifugation and SEC

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BMC Methods

ISSN: 3004-8729

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