Fig. 1

Workflow for preparing Mosquito Tissues using U-ExM (MoTissU-ExM). Workflow diagram for the dissection, preparation, and expansion of mosquito tissues. Note that the workflow alternates between being read left to right (steps 1. – 4. and 6. – 8.) and right to left (steps 5. And 9. to 11.) between rows. A detailed step-by-step text version of this process can be found in the methods section. Steps 3. – 11. depict salivary glands, but the process is the same for midguts. Longitudinal opening of midguts is not depicted in this workflow. PFA: paraformaldehyde, RT: room temperature: FA: formaldehyde, AA: acrylamide, O/N: overnight, DI H2O: deionised water, PBS: phosphate buffered saline, WGA: wheat germ agglutinin, PG: propyl gallate. Snowflakes indicate stages where gels can be cryopreserved at -20 °C