Fig. 5

Mosquito midgut U-ExM preserves both parasite and host ultrastructure. P. berghei infected mosquito midguts were prepared by U-ExM, or left unexpanded, stained with SYTOX (cyan, DNA) and NHS ester Alexa Fluor 405 (greyscale, protein density), and imaged by Airyscan Microscopy. From a larger section of the mosquito midgut (black), ultrastructural conservation of both parasite (oocyst, orange) and host (microvilli, green arrows & muscle fibres, magenta arrows) can be observed. Note that images of the unexpanded midgut are not the same midgut prepared by U-ExM. No significant gross, or ultrastructural abnormalities were observed in either the mosquito tissue or parasite following U-ExM. obj. = objective lens. Black scale bar = 100 µm, white scale bar = 20 µm, yellow scale bar = 10 µm. All images are a single z-slice