Fig. 6

Imaging of an infected midgut before and after MoTissU-ExM. a A mosquito midgut infected with P. berghei was fixed, stained with Hoechst (cyan, DNA) and NHS ester Alexa Fluor 405 (greyscale, protein density), and imaged by Airyscan microscopy. The entire midgut (top row), a section of that midgut (magenta), an individual oocyst (orange), and forming sporozoites in that oocyst (green) are all depicted. b After imaging, the same midgut, section, and oocyst were prepared by U-ExM, stained with SYTOX (cyan, DNA), re-stained with NHS Ester, and re-imaged. Number in the bottom corner of each image indicates z-projection depth in µm. The projections shown in the zoom panels are only representative of the chosen region of interest and do not display the full depth of the midgut. obj. = objective lens. Scale bars: black = 1000 µm, magenta = 50 µm, orange = 25 µm, green = 5 µm