Table 2 Summary of all fluorescent dyes and stains used in this study. *Indicates that dye was also used on unexpanded samples
Stain/dye | Source | Concentration | Step of protocol applied | Cat no |
|---|---|---|---|---|
NHS Ester Alexa Fluor 405 | ThermoFisher | 1:250 (2 mg/mL stock in DMSO) | Secondary Ab* | A30000 |
BODIPY-TR-Ceramide | ThermoFisher | 1:500 (1 mM stock in DMSO) | Post expansion | D7540 |
BODIPY-FL-C5-Ceramide | ThermoFisher | 1:500 (1 mM stock in DMSO) | Post expansion* | D3521 |
SYTOX Deep Red | ThermoFisher | 1:1000 (1 mM stock in DMSO) | Secondary Ab* | S11381 |
Wheat Germ Agglutinin Texas Red-X | ThermoFisher | 1:250 (1 mg/mL stock in PBS) | Post expansion | W21405 |
Hoechst 33,342 | ThermoFisher | 1:1000 (10 mg/mL stock in water) | Unexpanded only | C10329G |