Fig. 1

Preservation methods for human skeletal muscle biopsies: schematic and PSR-stained images. a Schematic representation of ideal myofibre arrangement and juxtaposition; representative PSR-stained images of human skeletal muscle tissue sections preserved with various methods are shown: b Direct embedding in OCT without fixation; c Fixation with 10 % formaldehyde, followed by embedding in OCT; d Refreezing with LN₂, followed by embedding in OCT; e Fixation with 36 % formaldehyde, followed by embedding in OCT. Panel a) was created using BioRender. Scale bar = 200 µm. The images are presented to illustrate artefacts observed