Fig. 2

Morphological analysis and schematic representation of myofibre disruption and ice crystal artefacts. a PSR-stained myofibres demonstrating endomysium disruption and separation of myofibres; b ORO-stained myofibres exhibiting the presence of intracellular vacuoles; c Fluorescence microscopy image stained with rhodamine-phalloidin (magenta) for actin and DAPI (cyan) for nuclei, revealing gaps within the myofibres, indicative of potential structural alterations; image has been made compatible for colourblind accessibility; d Schematic diagram generated using BioRender illustrating a cell with vacuoles/gaps to correlate with ice crystal artefacts. Scale bar = 50 µm