Table 1 Nine methods for HMW genomic DNA extraction from SAG 698-1b
No | Methods | Absorbance A260:280 | Absorbance A260:230 | Concentration (ng/μl) (Nanodrop) | Concentration (ng/μl) (Qubit) |
|---|---|---|---|---|---|
1 | GeneJET Plant Genomic DNA purification kit | 1.10 ± 0.002 | 0.57 ± 0.006 | 43.38 ± 2.32 | 0.56 ± 0.02 |
2 | NucleoSpin Plant II Mini kit | 1.39 ± 0.003 | 1.34 ± 0.01 | 134.58 ± 1.18 | 0.89 ± 0.01 |
3 | DNeasy Plant Mini kit | 1.10 ± 0.002 | 0.79 ± 0.01 | 51.01 ± 1.02 | 0.84 ± 0.01 |
4 | DNeasy PowerPlant Pro kit | 1.03 ± 0.02 | 0.51 ± 0.03 | 40.26 ± 3.22 | 9.12 ± 0.04 |
5 | CTAB method | 1.12 ± 0.001 | 0.54 ± 0.002 | 112.57 ± 1.82 | 2.55 ± 0.02 |
6 | CTAB/SDS/Phenol method | 1.04 ± 0.005 | 0.54 ± 0.006 | 226.15 ± 1.89 | 5.33 ± 0.03 |
7 | SDS method | 1.02 ± 0.002 | 0.75 ± 0.001 | 27.63 ± 0.33 | 1.72 ± 0.01 |
8 | Lithium chloride method | 1.17 ± 0.006 | 0.56 ± 0.006 | 171.72 ± 0.64 | 4.83 ± 0.35 |
9 | Nuclei isolation/ modified CTAB (our method) | 1.79 ± 0.005 | 1.90 ± 0.03 | 49.14 ± 1.23 | 38.07 ± 0.35 |