Fig. 1
From: Implementation and calibration of spectroscopic single-molecule localization microscopy
3D DWP sSMLM system detection and excitation path optical overview (not drawn to scale for clarity). a Single raw frame of a spatial image (Scale bar: 1.8 µm). b Single raw frame of a spectral image. c Spatial and spectral signatures of AF647 (green outline) and CF680 (red outline). Scale bar: 700 nm. d Adjustable illumination light path. M: mirror; DM: dichroic mirror; BE: Beam expander; L: lens, BPF: band-pass filter; Obj.: objective lens. e DWP sSMLM detection light path. LPF: long-pass filter; TL: tube lens; BS: Beam splitter; RP: right prism; DWP: dual-wedge prism. f Solidworks rendering of DWP assembly and its holder that slots into the sideport of the microscope body. g SEM image of the nanohole array, scale bar: 5 μm. h Magnified view of one nanohole highlighted in (d), Scale bar: 200 nm