Fig. 4

Typical imaging results. a 3D DWP 4-color image of a HeLa cell immunolabeled using antibodies to alpha-tubulin (DL680, red), vimentin (CF660C, green), Tom20 (AF647, gray), and PMP70 (DY-634, cyan), scale bar: 5 μm. b A normalized histogram of spectral peaks identified using the spectral fitting and matching method. c Isolated mitochondria from (a) filtered by the spectral region shown by the grey square in (b) (scale bar: 5 μm). d Isolated tubulin from (a) filtered by the spectral region shown by the red square in (b). e Isolated vimentin from (a) filtered by the spectral region shown by the green square in (b). f Isolated PMP70 from (a) filtered by the spectral region shown by the cyan square in (b). g Same image as in (c) with pseudocolors corresponding to the axial position. Colorbar: −350 to 350 nm (h) Same image as in (e) with pseudocolors corresponding to the axial position