Table 1 Data collection and processing parameters
From: Efficient strategies and troubleshooting for single particle cryoEM data collection using EPU
Parameters | Experiment 1 (EMD-48840) | Experiment 2 (EMD-48841) | Experiment 3 (EMD-48842) | Experiment 4 (EMD-48843) | Experiment 5 (EMD-48844) | Experiment 6 (EMD-48845) |
|---|---|---|---|---|---|---|
Pixel Size (Å) | 0.66 | 0.66 | 0.66 | 0.66 | 0.33 | 0.66 |
Acquisition mode | Counted Super resolution | Counted Super resolution | Counted Super resolution | Counted Super resolution | Counted Super resolution | Counted |
Mode | Accurate | Faster | Accurate | Faster | Faster | Faster |
Image format | TIFF | TIFF | MRC | MRC | TIFF | TIFF |
Binning | 2 | 2 | 2 | 2 | 1 | 1 |
Bit depth | 8 | 8 | 8 | 8 | 8 | 8 |
Images per hour | 134 | 736 | 160 | 758 | 338 | 307 |
Total number of images | 1354 | 1540 | 1582 | 1546 | 1542 | 1546 |
CTF average (Å) | ~ 2.5 | ~ 2.5 | ~ 2.5 | ~ 2.5 | ~ 2.5 | ~ 2.5 |
Number of squares selected | 5 | 5 | 5 | 5 | 5 | 7 |
Number of holes selected | 677 | 770 | 791 | 773 | 771 | 773 |
Each movie size | ~ 166 MB | ~ 166 MB | ~ 920 MB | ~ 920 MB | ~ 288 MB | ~ 166 MB |
Final number of particles | ~ 180,000 | ~ 180,000 | ~ 180,000 | ~ 180,000 | ~ 180,000 | ~ 180,000 |
Symmetry | Octahedral | Octahedral | Octahedral | Octahedral | Octahedral | Octahedral |
Resolution | 2.19 Å | 2.80 Å | 2.34 Å | 2.79 Å | 2.83 Å | 2.77 Å |
Resolution after beam shift correction | 2.14 Å | 2.09 Å | 2.12 Å | 2.12 Å | 2.16 Å | 2.30 Å |