Fig. 2

Immunostaining for Inflammatory versus Non-Inflammatory Phenotype in RAW264.7-IRCs. A Phenotyping via immunostaining of RAW264.7-IRCs for CD206 (green), a common anti-inflammatory marker, and CD86 (blue), a common inflammatory marker, co-localized with mRFP1 expression between no treatment and LPS treated cells (10 ng/mL). Scale bars are 20 µm. B Ratio of mean fluorescent intensities of CD86:CD206 to mRFP1 fluorescent intensity as measured by confocal microscopy. C. Phenotyping via flow cytometry of RAW264.7-IRCs for CD86 (left) and CD206 (right) alongside mRFP1 expression under three conditions: unstained, non-stimulated, and LPS treated (10 ng/mL). The black arrows showcase the magnitude of the cell population shift due to LPS stimulation