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Collaborative efforts, such as the Human Cell Atlas, are rapidly accumulating large amounts of single-cell data. To ensure that single-cell atlases are representative of human genetic diversity, we need to det...
Protein fractional turnover rates (FTR) represent measurements of flux through a protein pool, i.e., net abundance (ABD) of the protein. However, if protein abundance is not measured or varies between experime...
Macrophages are a promising target for therapeutics in various applications such as regenerative medicine and immunotherapy for cancer. Due to their plastic nature, macrophages can switch from a non-activated ...
Targeting undruggable proteins and challenging binding sites, such as protein–protein interaction (PPI) interfaces and allosteric pockets, using small-molecule inhibitors is often infeasible. Peptide-based irr...
Metatranscriptomics, which analyzes gene expression patterns in a heterogeneous community, is a powerful tool to evaluate microbial functional activity. A key challenge in this process is obtaining high-qualit...
Cells employ myriad regulatory mechanisms to maintain protein homeostasis, termed proteostasis, to ensure correct cellular function. Dysregulation of proteostasis, which is often induced by physiological stres...
Successful sequencing of genomes on Oxford Nanopore Technologies (ONT) sequencing platforms is dependant on obtaining sufficient amounts of high-quality genomic DNA. ONT’s Rapid PCR kit (SQK-RPB114.24) kit is ...
CryoEM has gained popularity due to advancements in both hardware and software, making it possible to visualize biological molecular structures in detail. However, despite its growing use, cryoEM remains a cos...
Spectroscopic single-molecule localization microscopy allows multi-color super-resolution images with high spectral sensitivity. While its profile has been steadily growing, it is still under-utilized in biolo...
To address the limitations of large-scale high quality microscopy image acquisition, PSSR (Point-Scanning Super-Resolution) was introduced to enhance easily acquired low quality microscopy data to a higher qua...
Duchenne Muscular Dystrophy (DMD) is a severe, progressive muscle-wasting disorder predominantly affecting boys, with an incidence of 1 in 5,000 births. It is caused by loss-of-function mutations in the X-linked
Functional cell processes (e.g., molecular signaling, response to stimuli, mitosis, etc.) impact cell phenotypes, which scientists can measure with cell morphology. However, linking these measurements with phe...
Skeletal muscle biopsies are valuable in clinical and research settings, contributing to advancements in diagnosing, understanding, and treating muscle-related conditions. Traditional freezing methods often ca...
Zygnematophyceae green algae represent the closest living relatives of land plants. Adaptions to hydro-terrestrial environments are evident through the production of mucilage carbohydrates, which are secreted ...
The use of single cell/nucleus RNA sequencing (scRNA-seq) technologies that quantitively describe cell transcriptional phenotypes is revolutionizing our understanding of cell biology, leading to new insights i...
It is known that the adenosine triphosphate (ATP) hydrolysis in a number of ATPases is reversible. Radioactive methods are standard in measuring ATPase reversibility. We used myosin as a well-studied model enz...
Study of malaria parasite cell biology is challenged by their small size, which can make visualisation of individual organelles difficult or impossible using conventional light microscopy. In recent years, the...
Spores are highly resistant dormant cells, adapted for survival and dispersal, that can withstand unfavourable environmental conditions for extended periods of time and later reactivate. Understanding the germ...
Bacterial high-copy number plasmids are the preferred DNA source for reporter constructs used in cell transfection experiments and making transgenic invertebrates to study gene expression, develop gene therapi...
Medical imaging techniques play a crucial role in diagnosing major diseases such as cardiovascular diseases, cancers, and neurological disorders. The BMC Methods Collection “Advances in medical imaging techniques...
Extracellular vesicles (EVs) represent small lipid bilayer structures pivotal in mediating intercellular communication via biomolecular transfer. Their inherent characteristics, including packaging, non-immuno...
Gene transcription by RNA polymerase II is a fundamental process in eukaryotic cells. The precise regulation of transcription is necessary for cellular growth and development and requires the coordinated activ...
Extracellular vesicles (EVs) are amongst the most promising candidates for developing blood-based biomarkers. However, patient sample availability is a key barrier to translational research whilst most biobank...
Primary tumour biology and axillary lymph node status are key prognostic factors in breast cancer treatment. The LowMag trial introduced a magnetic sentinel lymph node biopsy procedure using a superparamagneti...
Functional evaluation of molecules that are predicted to promote stem cell mediated endogenous repair often requires in vivo transplant studies that are low throughput and hinder the rate of discovery. To offe...
Recent technological advancements have vastly improved access to high-throughput biological instrumentation, sparking an unparalleled surge in omics data generation. The implementation of artificial intelligen...
Genome editing enables precise genetic manipulation in plants, offering hope for tackling global food insecurity and malnutrition by enhancing crop traits and nutritional content. The BMC Methods Collection ‘Geno...
Methods in tissue engineering and regenerative medicine are constantly evolving to address the complex challenges of repairing damaged tissues and modeling diseased organs using a library of various biomateria...
In the ever-evolving landscape of scientific research, the importance of detailed, accessible methods and step-by-step reusable protocols cannot be overstated. In alignment with this BMC Methods, is dedicated ...
